Why doesn't my FRT site logo invert when I reverse complement it? (not a serious complaint..) Signed up and played around a bit tonight. Really great concept. I think a couple of features will probably be helpful to your target audience, though some are a bit tricky to implement:
-User designed sequences (maybe I just couldn't find it). Everyone wants to drop their primer of interest in, their unique tag, their CRISPR site, etc. Also, some well-balanced GC spacers would be helpful, even make them unique so people could PCR off them if needed.
-Barcoded sequences. This will ruin / complicate the auto IDT pricing, but a nice drop-in feature would be a degenerate barcode sequence. This is all the range with MPRAs or other high-throughput methods, and could be really helpful
-Architectures. Say I want to design a lentivirus construct. Give the user the scaffold to drop their payload in, with set LTRs, etc. Screen for no polyA signals in their design, etc. An easier way to walk novice users into design (maybe best not to start with lenti...)
Anyway, keep up the good work, it's a cool product!
-User designed sequences (maybe I just couldn't find it). Everyone wants to drop their primer of interest in, their unique tag, their CRISPR site, etc. Also, some well-balanced GC spacers would be helpful, even make them unique so people could PCR off them if needed.
-Barcoded sequences. This will ruin / complicate the auto IDT pricing, but a nice drop-in feature would be a degenerate barcode sequence. This is all the range with MPRAs or other high-throughput methods, and could be really helpful
-Architectures. Say I want to design a lentivirus construct. Give the user the scaffold to drop their payload in, with set LTRs, etc. Screen for no polyA signals in their design, etc. An easier way to walk novice users into design (maybe best not to start with lenti...)
Anyway, keep up the good work, it's a cool product!